Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (1): 82-86.doi: 10.3969/j.issn.1673-8225.2011. 01.018

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Establishment of a mouse model of graft-versus-host disease

Zhu Jie-lin1, Zhang Peng1, Chen Fu2, Hou Ru-rong3   

  1. 1Department of Hematology, 3Department of Radiotherapy, 4Department of Laboratory, Zhongshan Hospital Affiliated to Xiamen University, Xiamen  361004, Fujian Province, China; 2Animal Laboratory, Medical College, Xiamen University, Xiamen  361005, Fujian Province, China
  • Received:2010-07-12 Revised:2010-10-26 Online:2011-01-01 Published:2011-01-01
  • Contact: Zheng Peng, Master, Chief physician, Department of Hematology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen 361004, Fujian Province, China xm2981@163.com
  • About author:Zhu Jie-lin★, Studying for master’s degree, Department of Hematology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen 361004, Fujian Province, China zhujielin.happy@163.com
  • Supported by:

    the Medical Innovation Project of Fujian Province in 2009, No. 2009-CXB-61

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Abstract:

BACKGROUND: Mouse graft-versus-host disease (GVHD) is the main complication of allogenic hematopoietic stem cell transplantation, presents the damages to multisystem (skin, esophago, stomach intestine, liver), and is one of reasons for death following allogenic hematopoietic stem cell transplantation.
OBJECTIVE: To explore the establishment of the mouse model of GVHD, and to provide effective animal models for experimental GVHD research.
METHODS: C57BL/6(H-2b)→BALB/C(H-2d) was selected as donor and recipient of complete allotransplantation. The GVHD models were established by injection of lymphocytes and bone marrow cells of the spleen of donor mouse. Whether GVHD models were established was assessed according to clinical situation and pathological examination.
RESULTS AND CONCLUSION: The recipient mice which accepted above 5×106 donor spleen cells developed acute GVHD after transplantation. However, the time when clinical situation of GVHD appeared and life span were different. Results indicated that the transfusion of 5×106 spleen cells was adequate to establish the mouse model of GVHD. The mouse model is reliable to the experimental research of GVHD.

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